Production, design, and evaluation of Bacillus thuringiensis serov. israelensis formulations against Aedes aegypti larvae

Bacillus thuringiensis israelensis (Bti) was cultured on three culture media: a reference medium and two new media. Then, spore-crystal complexes were recovered evaluated against Aedes aegypti larvae. After formulations, tablets granulates, prepared, then irradiated with ultraviolet light for larval mortality residual activities. The most toxic extract obtained in soluble 1 showing the lowest LC50 of 0.3748 mg/L. When Bti formulated tested laboratory, granulated presented approximately 90%–100% mortality, during first 3 days post-application, whereas showed 40%, unformulated 22% mortality. formulations reduced 5%–12% (tablets) granular (6%-11%) after 72 hours exposure to UV light; otherwise, reductions higher (32%).